The 70 S monosome accumulation and in vitro initiation complex formation by Escherichia coli ribosomes at 5 C

The inhibition of translation which is observed after shifting Escherichia coli to low temperature was investigated. 70 S ribosomes were isolated from E. coli 8 hours after a shift to 5 C synthesized protein in the absence of added mRNA (i.e., endogenous protein synthesis by 70 S monosomes) at a rate which was three times greater than the rate of endogenous protein synthesis by 70 S ribosomes which were isolated at the time of the shift to 5 C. Calculations based on the rates of endogenous protein synthesis and polyphenylalanine synthesis indicate that 70 S monosomes comprise only 0.1% of the total E. coli 70 S ribosome population after 8 hours at 5 c. Experiments designed to test initiation complex formation on ApUpG or formaldehyde treated MS-2 viral RNA demonstrated that, although the rate of formation of 30 S initiation complexes was not inhibited, the rate of formation of active 70 S initiation complexes, able to react with puromycin, was inhibited to a great extent at 5 C. A model depicting the effects of low temperature on the E. coli translation system is proposed

Opportunities for Dutch Biorefineries

Deze Roadmap Bioraffinage beschrijft een aantal mogelijke routes naar de ontwikkeling en implementatie van een bioraffinage-gerelateerde Bio-based Economy in Nederland. De Roadmap combineert korte- en middellange termijn mogelijkheden (commerciële implementatie, demonstratie plants, pilot plants en gerelateerd toegepast onderzoek) met strategisch onderzoek voor de langere termijn. Tevens zijn vier z.g. Moonshots uitgewerkt, als voorziene bioraffinagestrategieën met een grote potentie voor de Nederlandse economi

Master plan : Greenport Shanghai Agropark

Greenport Shanghai is the innovative and ambitious exploration of how Chinese metropolitan agriculture will jump into the 21st century: circular, sustainable and profitable

Prediction of an excessive response in in vitro fertilization from patient characteristics and ovarian reserve tests and comparison in subgroups: an individual patient data meta-analysis.

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Food Loss and Waste country profile for China - Estimates of Food Loss and Waste, associated GHG emissions and nutritional losses

The UN and the Champions 12.3 Coalition launched the ‘Target-Measure-Act approach’ calling on all governments and companies to set FLW reduction targets, measure FLW, identify hotspots, and to take action to reduce FLW accordingly (Lipinski, 2020). Lack of data makes it particularly difficult for low- and middle-income countries (LMIC) to specify the hotspot food products and chain stages, to define smart targets and to identify adequate interventions. In order to contribute to this essential information we developed and used a mass flow model based on secondary data (see next section for details). This approach allows to present an indicative country profile showing per food product category and chain stage not only the amount of FLW but also the GHGEs related to producing the FLW and induced nutrient losses. This integrated approach towards FLW reduction can support policy makers and other food system actors taking informed decisions contributing to several sustainability objectives in parallel

Food Loss and Waste country profile for Kenya - Estimates of Food Loss and Waste, associated GHG emissions and nutritional losses

The UN and the Champions 12.3 Coalition launched the ‘Target-Measure-Act approach’ calling on all governments and companies to set FLW reduction targets, measure FLW, identify hotspots, and to take action to reduce FLW accordingly (Lipinski, 2020). Lack of data makes it particularly difficult for low- and middle-income countries (LMIC) to specify the hotspot food products and chain stages, to define smart targets and to identify adequate interventions. In order to contribute to this essential information we developed and used a mass flow model based on secondary data (see next section for details). This approach allows to present an indicative country profile showing per food product category and chain stage not only the amount of FLW but also the GHGEs related to producing the FLW and induced nutrient losses. This integrated approach towards FLW reduction can support policy makers and other food system actors taking informed decisions contributing to several sustainability objectives in parallel